Test Vitamin D

Point-of-care | Quantitative Vitamin D test

The VHC Quantitative Vitamin D Test allow healthcare professionals to measure “point of care” vitamin D in few minutes. 

The test is an immunochromatography-based one step in vitro test. It is designed for the quantitative determination of total 25-hydroxy Vitamin D (25-OH Vitamin D) in human finger-prick blood. This assay provides a preliminary diagnostic test result and can be used for screening of Vitamin D deficiency.

VHC Vitamin-D Quantitative Vitamin D Test utilizes the principle of immunochromatography, a unique two-site “Sandwich” immunoassay on a membrane. The test employs a very “Exclusive” pair of anti-25-OH Vitamin D Monoclonal Antibodies; one conjugated with colloidal gold and another one immobilized on the solid phase. This will selectively detect Vitamin D with a high degree of sensitivity and specificity.

The liquid chromatography with tandem mass spectrometry (LC-MS/MS) assays or other quantitative immunoassays are recommended to further confirm the diagnostic test results.

Go to shop

Quantitative

Vitamin D test for in vitro diagnostics only.

Vitamin D easy to use test

Pain free collection and simple process to get a measurement of the patients Vitamin D level.

Chairside

Chairside test that allow you to give the patient an indication of their Vitamin D level, as you speak.

Flexible

Flexible reader technology enable read outs of several different tests.

Specimen collection and preparation

 

Step 1 

Pain free puncture with safety lancet

Step 2 

Adding light pressure a drop of blood emerge

Step 3 

The drop of blood is collected with the UniSampler™

Step 4 

Buffer liquid is added to the UniSampler™

Step 5 

In the UniSampler™ blood and buffer is mixed by a gentle shake

Step 6 

The mix of blood and buffer is placed on the test window (S)

Step 7

The total sample liquid should be 3-4 drops

Step 8

VHC Reader adaptor is placed on top of the test

Step 9

The VHC Reader is placed on the adaptor and the program is initiated.

Pictogram UniSampler™ Device

Performance characteristics

Sensitivity

The sensitivity of VHC Vitamin-D Quantitative Vitamin D Test is 3ng/ml (7.5nmol/l). The sensitivity was determined by calculating the mean plus 3.3 times of standard deviation of twenty Vitamin D-free serum tests.

Specificity

30 Vitamin D free serum samples were tested and all showed negative results; suggesting 100% specificity. No interference and cross reactivity was observed with Bilirubin, Triglycerides, Cholesterol, Vitamin B12 and Vitamin C.

Accuracy

The accuracy of VHC Vitamin-D Quantitative Vitamin D Test was evaluated using human finger-prick blood samples in comparison with a reference 25OH Vitamin D ELISA assay using corresponding serum samples. The comparison result showed a linear regression with slope of 1.02 and Correlation Coefficient of 92%. In conclusion, VHC Vitamin-D Quantitative Vitamin D Test results of human blood samples showed good agreement with the ELISA results of corresponding serum samples.
The accuracy of VHC Vitamin-D Quantitative Vitamin D Test was also evaluated using 20 serum samples in comparison with LC-MS/MS Assay (“Gold Standard” for 25-OH Vitamin D measurement). The comparison result showed a linear regression with the slope of 0.98 and Correlation Coefficient of 98%. In conclusion, VHC Vitamin-D Quantitative Vitamin D Test results agree closely to the true values generated from LC-MS/MS assay.

 

Precision

Sample No of replicates Mean ng/ml Standard deviation Coeficient Variation (CV)
Serum 20 40.4 3.1 7.6%
Blood-1 10 33 2.5 7.6%
Blood-2 10 38.7 3.9 10.1%

The complete package needed

click the edit button to change this text.

click the edit button to change this text.

click the edit button to change this text.

Get started testing Vitamin D “Point of Care”

Start-up packages

  1. 10 x Tests and a Reader, ready to use
  2. 25 x Tests and a Reader, ready to use
  3. 50 x Tests and a Reader, ready to use

Order & QA

Order by Go to shop

Questions: +45 3131 1925

Instructions for use

Download IFU here