Point-of-care | Quantitative Vitamin D test
The VHC Quantitative Vitamin D Test allow healthcare professionals to measure “point of care” vitamin D in few minutes.
The test is an immunochromatography-based one step in vitro test. It is designed for the quantitative determination of total 25-hydroxy Vitamin D (25-OH Vitamin D) in human finger-prick blood. This assay provides a preliminary diagnostic test result and can be used for screening of Vitamin D deficiency.
VHC Vitamin-D Quantitative Vitamin D Test utilizes the principle of immunochromatography, a unique two-site “Sandwich” immunoassay on a membrane. The test employs a very “Exclusive” pair of anti-25-OH Vitamin D Monoclonal Antibodies; one conjugated with colloidal gold and another one immobilized on the solid phase. This will selectively detect Vitamin D with a high degree of sensitivity and specificity.
The liquid chromatography with tandem mass spectrometry (LC-MS/MS) assays or other quantitative immunoassays are recommended to further confirm the diagnostic test results.
Go to shopPerformance characteristics
Sensitivity
The sensitivity of VHC Vitamin-D Quantitative Vitamin D Test is 3ng/ml (7.5nmol/l). The sensitivity was determined by calculating the mean plus 3.3 times of standard deviation of twenty Vitamin D-free serum tests.
Specificity
30 Vitamin D free serum samples were tested and all showed negative results; suggesting 100% specificity. No interference and cross reactivity was observed with Bilirubin, Triglycerides, Cholesterol, Vitamin B12 and Vitamin C.
Accuracy
Precision
| Sample | No of replicates | Mean ng/ml | Standard deviation | Coeficient Variation (CV) |
|---|---|---|---|---|
| Serum | 20 | 40.4 | 3.1 | 7.6% |
| Blood-1 | 10 | 33 | 2.5 | 7.6% |
| Blood-2 | 10 | 38.7 | 3.9 | 10.1% |
